BIOLOGICAL SAFETY MANUAL                                 

• Definition of Biohazards and Potentially Infectious Material

• Procedures for Biohazard Control

• Class II Biological Safety Cabinets

• Posting of Biological Hazard Signs

• Emergency Response
  a.  Biohazard Spills inside a Biological Safety Cabinet
  b.  Biohazard Spills outside a Biological Safety Cabinet
  c.  Decontamination and Clean Up of Blood Spills

• Biohazardous Waste
  a.  Definitions

• Procedures for the Safe Disposal of Biohazardous Waste

• Agents required to be autoclaved prior to disposal

• Decontamination, Sterilization and Disinfection

• Shipment of Biological Materials

• Cell Cultures

• Research Involving Recombinant DNA

• Recommended Biosafety Levels for Infectious Agents and Infected Animals
  a.  Table I - Summary of Recommended Biosafety Levels for Infectious Agents
  b.  Table II - Summary of Recommended Biosafety Levels for activities in which experimentally or naturally infected vertebrate animals are used

• Summary of Laboratory Practices for each Biosafety Level and Laboratory Biosafety Level Criteria

• Classification of Human Etiologic Agents on the Basis of Hazard
  a.  Oncogenic Viruses, Bacteria, Fungal Agents, Parasitic Agents, Viruses and Prions
  (Appendix B of the Recombinant DNA guidelines)