BIOLOGICAL SAFETY MANUAL
| 1. | Definition of Biohazards and Potentially Infectious Material | |
| 2. | Procedures for Biohazard Control | |
| 3. | Class II Biological Safety Cabinets | |
| 4. | Posting of Biological Hazard Signs | |
| 5. | ||
| a. Biohazard Spills inside a Biological Safety Cabinet | ||
| b. Biohazard Spills outside a Biological Safety Cabinet | ||
| c. Decontamination and Clean Up of Blood Spills | ||
| 6. | Risk Assessments | |
| 7. | Laboratory Security Policy | |
| 8. | Biohazardous Waste | |
| a. Definitions | ||
| 9. | Procedures for the Safe Disposal of Biohazardous Waste | |
| 10. | Agents required to be Autoclaved Prior to Disposal | |
| 11. | Decontamination, Sterilization and Disinfection | |
| a. General | ||
| b. Definitions | ||
| c. Methods | ||
| 12. | Activity Levels of Selected Liquid Germicides | |
| 13. | Shipment of Biological Materials | |
| a. Table 1: Category A Infectious Substances | ||
| b. Table 2: Shipping Summary Table | ||
| c. Packing Instructions 602 Summary | ||
| d. Packing Instructions 650 Summary | ||
| e. Packing Instructions 904 Summary | ||
| f. Packing Instructions 913 Summary | ||
| g. Exempt Packing Instructions | ||
| 14. | Tissue Culture/Cell Lines | |
| 15. | Research Involving Recombinant DNA | |
| 16. | Summary of Recommended Biosafety Levels for Infectious Agents and Infected Animals | |
| a. Table 1 - Summary of Recommended Biosafety Levels for Infectious Agents | ||
| b. Table II - Summary of Recommended Biosafety Levels for activities in which experimentally or naturally infected vertebrate animals are used | ||
| 17. | Laboratory Practices for each Biosafety Level and Biosafety Level Criteria | |
| 18. | Vertebrate Animal Biosafety Level Criteria for Vivarium Research Facilities | |
| 19. | Classification of Human Etiologic Agents on the Basis of Hazard | |
| a. Oncogenic Viruses, Bacteria, Fungal Agents, Parasitic Agents, Viruses and Prions (Appendix B of the Recombinant DNA guidelines) | ||
| 20. | Recommended Procedures for Preventing the Production of Aerosols | |
| a. General | ||
| b. Centrifuge Operation | ||
| c. Mixing Operations | ||
| d. Vacuum and Aspirating Equipment | ||
| e. Needles and Syringes | ||
| f. Pipettes | ||
| g. Transfer Loops | ||
| 21. | Recommended Procedures for the Proper Use of Biological Safety Cabinets | |
| 22. | Recommended Procedures for Autoclaving Materials |
| University of the Sciences in Philadelphia • 600 South Forty-third Street • Philadelphia, PA 19104-4495 • phone: 215-596-8800 • email: safety@usp.edu |