Plant Tissue Culture (BS 332)

  

Mr. Robert A. Smith
STC 275
215/596/8918
r.smith@usip.edu   

haploid tobacco (24 chromosomes)

Course description
Plant Tissue Culture is a two credit course offered during the Spring semester. There is one lecture and three laboratory hours each week. This course is an introduction to the theory, practice and biotechnical applications of culturing plant cells, tissues, and organs. Case studies emphasize science process skills of problem posing, problem solving and presentation. Students use statistical, word processing, and spread-sheet programs.

Students must have taken BS 104 or BS 131 prior to registering for Plant Tissue Culture.

Lecture Syllabus   (Tuesdays 9:00 - 9:50 AM, STC 302)
Week 1       Introduction
The Process of Science
Case Study 1		  
Week 2   Aseptic technique, disinfection, sterilization  
Week 3      Tissue culture media - I  
Week 4 Tissue culture media - II  
Week 5    Callus Culture  
Week 6      Organ Culture  
Week 7 Embryogenesis
Case Study 2		  
Week 8 Organogenesis
Case Study 3		  
Week 9       Cell suspension culture  
Week 10       Single cell culture  
Week 11       Plant protoplasts  
Week 12 Mutagenesis and in-vitro selection  
Week 13 Production of isogenic lines  
Week 14  Secondary products  


Laboratory Schedule    (Tuesdays 10 AM -12:50 PM, STC 302)
Week 1 Ex 2

Student t test
Week 2 Ex 8

Disinfection of plant material

Week 3 Ex 20
Ex 21		 Autoclave sterilized media
Filter sterilized media
Week 4 Ex 22
Ex 9
Ex 10
 Bioassay of cytokinins
sweet potato root callus cultures
carrot root callus cultures
Week 5 Ex 14 Shoot-tip cultures
Student investigation
Week 6 Ex 16 Somatic embryogenesis
Week 7 Ex 17 Organogenesis
Week 8   Exam I
Week 9 Ex 12 Suspension cultures
Week 10 Ex 25 Isolation of oat leaf protoplasts
Week 11 handout Induction of secondary products
Week 12   Read results
Week 13   Read results
Week 14   Read results
 

Text
Smith, R. A. Plant Tissue Culture Studies. 1997. National Association of Biology Teachers: Reston, Virginia.
Students will use the primary literature for their investigative projects.

Laboratory Equipment
Lab coat
Notebook
black permanent marker

 Grading
Course grade will be based upon midterm and final examinations, a laboratory notebook, and a student project. Some exam questions require use of statistical programs.

Attendance
Attendance in lecture is recomended but not required. Attendence in lab is required. There are 14 weeks of lab. Any lab abscences must be made up. A $25.00 make-up fee, for each lab missed, must be paid to the Cashier before the laboratory can be made up, regardless of the reasons for the missed lab. There are no exceptions for any reason. A copy of the receipt must be presented to the instructor when making arrangements for a make-up lab time. If any lab is not made up, a grade of F will be assigned for the course. Students with 3 or more abscences, regardless of whether those labs have been made up, will fail ( grade of F) the course.

 

Grade equivalency

97-100 A+
93-96 A
90-92 A-
87-89 B+
83-86 B
80-82 B-
77-79 C+
73-76 C 		70-72 C-
67-69 D+
63-66 D
60-62 D-
< 60 F

  
 
Sample Data Analyses *

   normal distribution    t-test   correlation    chi-square    plotting

learning objectives

*(Provided by http://www.biology4all.com/resources_library/resources_library.asp)

USP home page
Created by: R.  A. Smith r.smith@usip.edu
Last update: January 2007.